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CD28 antibody (AA 1-120)

Name: CD28 antibody (AA 1-120)
SKU: ABIN674604
Price: 384.62 USD
Availability: InStock

CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse splenocytes probed with Rabbit Anti-CD28 Polyclonal Antibody, Unconjugated . (CD28 antibody (AA 1-120))

FACS

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD28 antibody (AA 1-120))

IHC

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD28 antibody (AA 1-120))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded Human rectal cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6. (CD28 antibody (AA 1-120))

IHC (p)

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD28 antibody (AA 1-120))

IHC

Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-CD28 Polyclonal Antibody, Unconjugated (ABIN674604) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD28 antibody (AA 1-120))

Paraformaldehyde-fixed, paraffin embedded Human stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6. (CD28 antibody (AA 1-120))

IHC (p)

7 images

(1 reference)

Catalog No. ABIN674604

Datasheet as PDF

Target See all CD28 Antibodies

CD28
Binding Specificity
15

15

12

9

8

5

3

3

2

2

1

1

1

1

1

1

1

1

1
AA 1-120
Reactivity
168

118

54

34

22

16

12

12

7

7

5

5

1

1

1

1
Human, Mouse, Rat
Host
127

76

28

16

8

3

2
Rabbit
Compatible Secondaries
Clonality
183

71

3
Polyclonal
Conjugate
88

31

28

21

14

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This CD28 antibody is un-conjugated
Application
197

89

53

47

37

32

28

25

18

17

12

7

6

5

4

4

4

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig,Horse,Chicken

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse CD28

Isotype

IgG
anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) (AA 19-152) antibody
CD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated

anti-CD28 (CD28) (C-Term) antibody
CD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE

anti-CD28 (CD28) (Internal Region) antibody
Verified CD28 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated

anti-CD28 (CD28) antibody (APC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PE

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Liu, He, Chen, Ding, Geng, Wu, Liu, Wang: "Costimulatory molecule CD28 participates in the process of embryo implantation in mice." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 21, Issue 6, pp. 686-95, (2014) (PubMed).
Target

CD28

Alternative Name

CD28 (CD28 Products)

Synonyms

Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background

Synonyms: T-cell-specific surface glycoprotein CD28, CD28
Background: Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Gene ID

12487

UniProt

P31041

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response