Fascin antibody (AA 93-142)

Catalog No. ABIN6746004

Product Details anti-Fascin Antibody

Target: Fascin (FSCN1)

Binding Specificity: AA 93-142

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog, Horse, Zebrafish (Danio rerio), Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse FSCN1 / Fascin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig (100%) Bovine (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa93-142 of mouse Fscn1 (Q61553, NP_032010). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Horse, Pig, Guinea pig (100%), Panda, Bovine, Opossum (92%), Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-Fascin antibody (ABIN6746004)

Dalle, Imamura, Rose, Worrall, Ugi, Hupfeld, Olefsky: "Insulin induces heterologous desensitization of G-protein-coupled receptor and insulin-like growth factor I signaling by downregulating beta-arrestin-1." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6272-85, (2002) (PubMed).

Attramadal, Arriza, Aoki, Dawson, Codina, Kwatra, Snyder, Caron, Lefkowitz: "Beta-arrestin2, a novel member of the arrestin/beta-arrestin gene family." in: The Journal of biological chemistry, Vol. 267, Issue 25, pp. 17882-90, (1992) (PubMed).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: FSCN1 / Fascin (FSCN1 Products)

Background: Name/Gene ID: FSCN1

Synonyms: FSCN1, 55 kDa actin-bundling protein, Fascin, HSN, Singed-like protein, SNL, p55

Gene ID: 6624

NCBI Accession: NP_032010

UniProt: Q16658