K-RAS antibody (N-Term)

Catalog No. ABIN604770

Product Details anti-K-RAS Antibody

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Dog, Zebrafish (Danio rerio), Rabbit, Horse, Guinea Pig, Cow, Drosophila melanogaster, Pig, Bat, Chicken, Xenopus laevis, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human KRAS

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human KRAS (P01111, NP_203524). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Frog, Catfish, Salmon, Smelt, Medaka, Pufferfish, Drosophila (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS (KRAS Products)

Target Type: Viral Protein

Background: Name/Gene ID: KRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: KRAS, C-K-RAS, C-Ki-ras, C-Kirsten-ras protein, GTPase KRas, K-ras p21 protein, K-RAS2B, K-RAS2, KRAS2, NS, NS3, K-RAS4A, KRAS1, RASK2, Transforming protein p21, Oncogene KRAS2, K-Ras 2, K-RAS2A, K-RAS4B, KI-RAS, PR310 c-K-ras oncogene

Gene ID: 3845

NCBI Accession: NP_203524

UniProt: P01116