DBT antibody (AA 35-84)

Catalog No. ABIN6745750

Product Details anti-DBT Antibody

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Binding Specificity: AA 35-84

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DBT / E2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig (100%) Chicken, Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human DBT (P11182, NP_001909). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Zebrafish, Beetle (92%), Trout, Drosophila (91%), Stickleback, Sea squirt (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: DBT / E2 (DBT Products)

Synonyms: BCATE2 antibody, BCKAD-E2 antibody, BCKADE2 antibody, E2 antibody, E2B antibody, D3Wsu60e antibody, im:7147214 antibody, zgc:103768 antibody, E2b antibody, dihydrolipoamide branched chain transacylase E2 antibody, hypothetical protein antibody, dihydrolipoamide branched chain transacylase E2 L homeolog antibody, DBT antibody, dbt antibody, CpipJ_CPIJ006326 antibody, BDBG_05874 antibody, NAEGRDRAFT_78509 antibody, VDBG_04820 antibody, PGTG_17722 antibody, Dbt antibody, dbt.L antibody

Target Type: Viral Protein

Background: Name/Gene ID: DBT

Synonyms: DBT, BCKAD E2 subunit, BCKAD-E2, BCKADE2, Dihydrolipoyl transacylase, E2, E2B, BCATE2

Gene ID: 1629

NCBI Accession: NP_001909

UniProt: P11182