Leptin antibody (C-Term)
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- Target See all Leptin (LEP) Antibodies
- Leptin (LEP)
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Cow, Horse, Dog, Goat, Sheep, Chicken, Hamster, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Leptin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human LEP / Leptin
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Bovine, Goat, Horse, Chicken (100%) Rabbit, Pig (92%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from C-Terminus of human LEP (P41159, NP_000221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Hamster, Elephant, Zebu, Bovine, Horse, Turkey, Chicken (100%), Orangutan, Cat, Rabbit, Pig (92%), Panda (85%), Marmoset (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LEP Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- Leptin (LEP)
- Alternative Name
- Leptin (LEP Products)
- Synonyms
- ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody
- Background
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Name/Gene ID: LEP
Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB - Gene ID
- 3952
- NCBI Accession
- NP_000221
- UniProt
- P41159
- Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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