SIRT5 antibody (AA 179-228)

Catalog No. ABIN6745720

Product Details anti-SIRT5 Antibody

Target: SIRT5 (Sirtuin 5 (SIRT5))

Binding Specificity: AA 179-228

Reactivity: Human, Mouse, Cow, Goat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SIRT5

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa179-228 of human SIRT5 (Q9NXA8, NP_036373). Percent identity by BLAST analysis: Human, Monkey (100%), Gorilla, Orangutan, Gibbon, Marmoset (92%), Rabbit, Pig (85%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIRT5

Target: SIRT5 (Sirtuin 5 (SIRT5))

Alternative Name: SIRT5 / Sirtuin 5 (SIRT5 Products)

Synonyms: SIR2L5 antibody, 0610012J09Rik antibody, 1500032M05Rik antibody, AV001953 antibody, zgc:92288 antibody, sirt5-a antibody, SIRT5 antibody, sir2l5 antibody, sirtuin 5 antibody, sirtuin 5 L homeolog antibody, SIRT5 antibody, Sirt5 antibody, sirt5 antibody, sirt5.L antibody, CC1G_00083 antibody

Background: Name/Gene ID: SIRT5
Family: ADP-Ribosyltransferase

Synonyms: SIRT5, SIR2L5, Sir2-like 5, SIR2-like protein 5, Sirtuin 5, Sirtuin type 5

Gene ID: 23408

NCBI Accession: NP_036373

UniProt: Q9NXA8

Pathways: SARS-CoV-2 Protein Interactome