RAD51 antibody (AA 35-80)

Catalog No. ABIN6745710

Product Details anti-RAD51 Antibody

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Binding Specificity: AA 35-80

Reactivity: Human, Mouse, Rat, Cow, Monkey, Rabbit, Guinea Pig, Horse, Pig, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RAD51

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Pig (100%) Dog, Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-80 of human RAD51 (Q06609-2, NP_597994). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig, Platypus, Xenopus (100%), Dog, Opossum, Turkey, Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51 / RECA (RAD51 Products)

Background: Name/Gene ID: RAD51

Synonyms: RAD51, BRCC5, HsT16930, RAD51 homolog A, RAD51 homolog (S. cerevisiae), RecA, E. coli, homolog of, RecA-like protein, Recombination protein A, MRMV2, HRAD51, HsRad51, RAD51A, RECA

Gene ID: 5888

NCBI Accession: NP_597994

UniProt: Q06609

Pathways: DNA Damage Repair