MED25 antibody (AA 33-82)

Catalog No. ABIN6745504

Product Details anti-MED25 Antibody

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Binding Specificity: AA 33-82

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED25 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human MED25

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Xenopus, Zebrafish (100%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa33-82 of human MED25 (Q71SY5, NP_112235). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Pig, Xenopus, Stickleback, Zebrafish (100%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED25

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Alternative Name: MED25 (MED25 Products)

Background: Name/Gene ID: MED25

Synonyms: MED25, ACID1, CMT2B2, TCBAP0758, Mediator complex subunit 25, PTOV2, ARC92, p78

Gene ID: 81857

NCBI Accession: NP_112235

UniProt: Q71SY5

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response