POLR1E antibody (AA 35-84)

Catalog No. ABIN6745190

Product Details anti-POLR1E Antibody

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Binding Specificity: AA 35-84

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1E antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human POLR1E / PRAF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig (100%) Rabbit, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human POLR1E (Q9GZS1-2, NP_071935). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Guinea pig (100%), Bat, Rabbit, Chicken (92%), Xenopus (91%), Zebra finch, Platypus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E (POLR1E Products)

Synonyms: 53kDa antibody, AU042259 antibody, D030019D19Rik antibody, Paf53 antibody, Praf1 antibody, PAF53 antibody, PRAF1 antibody, RP11-405L18.3 antibody, RNA polymerase I subunit E antibody, polymerase (RNA) I polypeptide E antibody, polymerase (RNA) I polypeptide E L homeolog antibody, POLR1E antibody, polr1e antibody, Polr1e antibody, polr1e.L antibody

Background: Name/Gene ID: POLR1E

Synonyms: POLR1E, PRAF1, RNA polymerase I subunit A49, PAF53, RP11-405L18.3

Gene ID: 64425

NCBI Accession: NP_071935

UniProt: Q9GZS1