WIF1 antibody (AA 71-120)

Catalog No. ABIN6744960

Product Details anti-WIF1 Antibody

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Binding Specificity: AA 71-120

Reactivity: Human, Mouse, Horse, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WIF1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human WIF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine, Rabbit, Horse (100%) Rat, Dog, Pig, Xenopus (92%) Zebrafish (83%) Chicken (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human WIF1 (Q9Y5W5, NP_009122). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Elephant, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Marmoset, Rat, Panda, Dog, Pig, Platypus, Xenopus (92%), Turkey (90%), Lizard (85%), Zebrafish (83%), Chicken (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for WIF1

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Alternative Name: WIF1 / WIF-1 (WIF1 Products)

Synonyms: shf antibody, GB10372 antibody, wif-1 antibody, WIF1 antibody, WIF-1 antibody, AW107799 antibody, zgc:110507 antibody, protein shifted antibody, wnt inhibitory factor 1 antibody, WNT inhibitory factor 1 antibody, Wnt inhibitory factor 1 antibody, WNT inhibitory factor 1 S homeolog antibody, LOC552805 antibody, CpipJ_CPIJ003761 antibody, wif1 antibody, WIF1 antibody, Wif1 antibody, LOC100511326 antibody, wif1.S antibody

Background: Name/Gene ID: WIF1

Synonyms: WIF1, WNT inhibitory factor 1, WIF-1

Gene ID: 11197

NCBI Accession: NP_009122

UniProt: Q9Y5W5

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation