FUBP3 antibody (AA 503-552)

Catalog No. ABIN6744454

Product Details anti-FUBP3 Antibody

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Binding Specificity: AA 503-552

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FUBP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FUBP3 / FBP3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig (92%) Mouse, Rat, Dog (85%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa503-552 of human FUBP3 (Q96I24, NP_003925). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Monkey, Galago, Marmoset, Elephant, Bovine, Bat, Horse, Pig (92%), Mouse, Rat, Dog (85%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FUBP3

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Alternative Name: FUBP3 (FUBP3 Products)

Synonyms: FBP3 antibody, Marta2 antibody, A330051M14Rik antibody, AV006371 antibody, zgc:101598 antibody, far upstream element binding protein 3 antibody, far upstream element-binding protein 3 antibody, far upstream element binding protein 3 S homeolog antibody, far upstream element (FUSE) binding protein 3 antibody, FUBP3 antibody, LOC100196681 antibody, Fubp3 antibody, fubp3.S antibody, fubp3 antibody

Background: Name/Gene ID: FUBP3

Synonyms: FUBP3, FBP3, FUSE-binding protein 3

Gene ID: 8939

NCBI Accession: NP_003925

UniProt: Q96I24