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HMGCS1 antibody (AA 251-300)

HMGCS1 Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Pig, Hamster, Xenopus laevis WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6744452
  • Target See all HMGCS1 Antibodies
    HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))
    Binding Specificity
    • 15
    • 15
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 251-300
    Reactivity
    • 40
    • 35
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Pig, Hamster, Xenopus laevis
    Host
    • 59
    • 4
    • 1
    Rabbit
    Clonality
    • 63
    • 1
    Polyclonal
    Conjugate
    • 31
    • 6
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HMGCS1 antibody is un-conjugated
    Application
    • 42
    • 22
    • 13
    • 13
    • 11
    • 8
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human HMGCS1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig (100%) Guinea pig (92%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa251-300 of human HMGCS1 (Q01581, NP_002121). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%), Marmoset, Bat, Guinea pig (92%), Opossum (85%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product HMGCS1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))
    Alternative Name
    HMGCS1 / HMG-CoA Synthase 1 (HMGCS1 Products)
    Synonyms
    HMGCS antibody, zgc:56481 antibody, B130032C06Rik antibody, 3-hydroxy-3-methylglutaryl-CoA synthase 1 antibody, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) antibody, 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 antibody, 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody, hydroxymethylglutaryl-CoA synthase antibody, 3-hydroxy-3-methylglutaryl-CoA synthase antibody, hydroxymethylglutaryl coenzyme A synthase antibody, 3-hydroxy-3-methylglutaryl-CoA synthase 1 S homeolog antibody, HMGCS1 antibody, hmgcs1 antibody, Hmgcs1 antibody, mvaS antibody, SAS2432 antibody, SZO_RS04705 antibody, SEQ_1109 antibody, NAEGRDRAFT_77778 antibody, hmgcs1.S antibody
    Background
    Name/Gene ID: HMGCS1

    Synonyms: HMGCS1, HMG-CoA synthase, HMGCS
    Gene ID
    3157
    NCBI Accession
    NP_002121
    UniProt
    Q01581
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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