TRPM2 antibody (AA 431-480)
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- Target See all TRPM2 Antibodies
- TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
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Binding Specificity
- AA 431-480
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Reactivity
- Human, Mouse, Rat, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TRPM2 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human TRPM2
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Horse (92%) Guinea pig (87%) Chicken (84%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa431-480 of human TRPM2 (O94759, NP_003298). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat (100%), Galago, Marmoset, Elephant, Bovine, Bat, Horse (92%), Guinea pig (87%), Panda, Platypus, Lizard (85%), Turkey, Zebra finch, Chicken (84%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product TRPM2 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (10 - 20 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
- Alternative Name
- TRPM2 (TRPM2 Products)
- Synonyms
- EREG1 antibody, KNP3 antibody, LTRPC2 antibody, NUDT9H antibody, NUDT9L1 antibody, TRPC7 antibody, Trpm2-predicted antibody, TRPM2 antibody, si:ch73-263b18.1 antibody, 9830168K16Rik antibody, C79133 antibody, Trp7 antibody, Trrp7 antibody, transient receptor potential cation channel subfamily M member 2 antibody, transient receptor potential cation channel, subfamily M, member 2 antibody, transient receptor potential cation channel subfamily C member 7 antibody, TRPM2 antibody, Trpm2 antibody, trpm2 antibody, TRPC7 antibody
- Background
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Name/Gene ID: TRPM2
Subfamily: Calcium channel - TRP
Family: Ion Channel
Synonyms: TRPM2, EREG1, LTrpC-2, LTRPC2, NUDT9L1, KNP3, NUDT9H, Trrp7 - Gene ID
- 7226
- NCBI Accession
- NP_003298
- UniProt
- O94759
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