GNAS antibody (AA 36-85)

Catalog No. ABIN490056

Product Details anti-GNAS Antibody

Target: GNAS (GNAS Complex Locus (GNAS))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Horse, Guinea Pig, Monkey, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GNAS

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human GNAS (P63092, NP_000507). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GNAS

Target: GNAS (GNAS Complex Locus (GNAS))

Alternative Name: GNAS (GNAS Products)

Synonyms: AHO antibody, C20orf45 antibody, GNAS1 antibody, GPSA antibody, GSA antibody, GSP antibody, NESP antibody, PHP1A antibody, PHP1B antibody, PHP1C antibody, POH antibody, GNAS antibody, PORGSA1 antibody, G-alpha-S antibody, 5530400H20Rik antibody, A930027G11Rik antibody, C130027O20Rik antibody, Galphas antibody, Gnas1 antibody, Gnasxl antibody, Gsa antibody, Nesp antibody, Nespl antibody, Oed-Sml antibody, Oedsml antibody, P1 antibody, P2 antibody, P3 antibody, G-alpha-s antibody, gnas-a antibody, Gnpas antibody, Nesp55 antibody, gnal antibody, Gnas antibody, GNAS complex locus antibody, guanine nucleotide-binding protein G(s) subunit alpha antibody, GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus antibody, GNAS complex locus S homeolog antibody, neuroendocrine secretory protein 55-like antibody, GNAS antibody, LOC694289 antibody, LOC469986 antibody, Gnas antibody, gnas.S antibody, gnas antibody, LOC100346323 antibody, LOC100442753 antibody, LOC100732436 antibody

Background: Name/Gene ID: GNAS

Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI

Gene ID: 2778

NCBI Accession: NP_000507

UniProt: Q5JWF2

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis