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GNAS antibody (AA 36-85)

GNAS Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Horse, Guinea Pig, Monkey, Hamster, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN490056
  • Target See all GNAS Antibodies
    GNAS (GNAS Complex Locus (GNAS))
    Binding Specificity
    • 17
    • 10
    • 8
    • 8
    • 7
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 36-85
    Reactivity
    • 85
    • 28
    • 24
    • 9
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog, Cow, Pig, Horse, Guinea Pig, Monkey, Hamster, Bat
    Host
    • 80
    • 9
    • 1
    Rabbit
    Clonality
    • 82
    • 8
    Polyclonal
    Conjugate
    • 50
    • 9
    • 8
    • 7
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GNAS antibody is un-conjugated
    Application
    • 74
    • 42
    • 32
    • 18
    • 17
    • 9
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human GNAS
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig (100%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa36-85 of human GNAS (P63092, NP_000507). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product GNAS Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    GNAS (GNAS Complex Locus (GNAS))
    Alternative Name
    GNAS (GNAS Products)
    Synonyms
    AHO antibody, C20orf45 antibody, GNAS1 antibody, GPSA antibody, GSA antibody, GSP antibody, NESP antibody, PHP1A antibody, PHP1B antibody, PHP1C antibody, POH antibody, GNAS antibody, PORGSA1 antibody, G-alpha-S antibody, 5530400H20Rik antibody, A930027G11Rik antibody, C130027O20Rik antibody, Galphas antibody, Gnas1 antibody, Gnasxl antibody, Gsa antibody, Nesp antibody, Nespl antibody, Oed-Sml antibody, Oedsml antibody, P1 antibody, P2 antibody, P3 antibody, G-alpha-s antibody, gnas-a antibody, Gnpas antibody, Nesp55 antibody, gnal antibody, Gnas antibody, GNAS complex locus antibody, guanine nucleotide-binding protein G(s) subunit alpha antibody, GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus antibody, GNAS complex locus S homeolog antibody, neuroendocrine secretory protein 55-like antibody, GNAS antibody, LOC694289 antibody, LOC469986 antibody, Gnas antibody, gnas.S antibody, gnas antibody, LOC100346323 antibody, LOC100442753 antibody, LOC100732436 antibody
    Background
    Name/Gene ID: GNAS

    Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI
    Gene ID
    2778
    NCBI Accession
    NP_000507
    UniProt
    Q5JWF2
    Pathways
    Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis
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