HOXC8 antibody (AA 35-84)

Catalog No. ABIN6744445

Product Details anti-HOXC8 Antibody

Target: HOXC8 (Homeobox C8 (HOXC8))

Binding Specificity: AA 35-84

Reactivity: Human, Mouse, Rat, Sheep, Dog, Cow, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOXC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HOXC8

Predicted Reactivity: Percent identity by BLAST analysis: Rat, Sheep, Dog, Bovine, Rabbit (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human HOXC8 (P31273, NP_073149). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit (100%), Pufferfish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HOXC8

Target: HOXC8 (Homeobox C8 (HOXC8))

Alternative Name: HOXC8 (HOXC8 Products)

Background: Name/Gene ID: HOXC8

Synonyms: HOXC8, Homeo box C8, Homeobox protein Hox-C8, Hox-3.1, mouse, homolog of, HOX3A, Homeo box 3A, Homeobox C8, Homeobox protein Hox-3A, HOX3

Gene ID: 3224

NCBI Accession: NP_073149

UniProt: P31273