ZNF71 antibody (C-Term)

Catalog No. ABIN6744444

Product Details anti-ZNF71 Antibody

Target: ZNF71 (Zinc Finger Protein 71 (ZNF71))

Binding Specificity: C-Term

Reactivity: Human, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF71 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ZNF71

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ZNF71 (Q9NQZ8, NP_067039). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Horse (100%), Gibbon, Mouse, Rat, Bovine, Bat, Guinea pig, Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ZNF71

Target: ZNF71 (Zinc Finger Protein 71 (ZNF71))

Alternative Name: ZNF71 (ZNF71 Products)

Synonyms: EZFIT antibody, zinc finger protein 71 antibody, uncharacterized LOC509356 antibody, ZNF71 antibody, LOC509356 antibody

Background: Name/Gene ID: ZNF71
Family: Zinc Finger

Synonyms: ZNF71, Cos26, Zinc finger protein 71, Zinc finger protein 71 (Cos26), EZFIT

Gene ID: 58491

NCBI Accession: NP_067039

UniProt: Q9NQZ8