DDAH2 antibody (N-Term)

Catalog No. ABIN6744442

Product Details anti-DDAH2 Antibody

Target: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDAH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DDAH2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human DDAH2 (O95865, NP_039268). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bat, Horse (100%), Monkey, Bovine (92%), Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DDAH2

Target: DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

Alternative Name: DDAH2 / DDAH (DDAH2 Products)

Synonyms: ddah2 antibody, MGC84290 antibody, g6a antibody, ddah antibody, ng30 antibody, ddahii antibody, MGC89103 antibody, DDAH2 antibody, DDAH antibody, DDAHII antibody, G6a antibody, NG30 antibody, 1110003M04Rik antibody, AU019324 antibody, AW413173 antibody, DDAH-2 antibody, Ddah antibody, dimethylarginine dimethylaminohydrolase 2 antibody, dimethylarginine dimethylaminohydrolase 2 L homeolog antibody, ddah2 antibody, ddah2.L antibody, DDAH2 antibody, Ddah2 antibody

Background: Name/Gene ID: DDAH2

Synonyms: DDAH2, DDAH, DDAH-2, DDAHII, G6a, Alternative name: NG30, G6a, Unknown, Dimethylargininase-2, NG30, Protein G6a, S-phase protein

Gene ID: 23564

NCBI Accession: NP_039268

UniProt: O95865