PHACTR1 antibody (AA 468-517)

Catalog No. ABIN6744441

Product Details anti-PHACTR1 Antibody

Target: PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))

Binding Specificity: AA 468-517

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Horse, Xenopus laevis, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHACTR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PHACTR1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa468-517 of human PHACTR1 (Q9C0D0, NP_112210). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%), Turkey (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PHACTR1

Target: PHACTR1 (Phosphatase and Actin Regulator 1 (PHACTR1))

Alternative Name: PHACTR1 (PHACTR1 Products)

Synonyms: DDSG1 antibody, RPEL antibody, RPEL1 antibody, dJ257A7.2 antibody, 9630030F18Rik antibody, Rpel1 antibody, phosphatase and actin regulator 1 S homeolog antibody, phosphatase and actin regulator 1 antibody, phactr1.S antibody, PHACTR1 antibody, Phactr1 antibody, LOC100726748 antibody

Background: Name/Gene ID: PHACTR1

Synonyms: PHACTR1, DJ257A7.2, RPEL1, RPEL, RPEL repeat containing 1, KIAA1733

Gene ID: 221692

NCBI Accession: NP_112210

UniProt: Q9C0D0