MKNK2 antibody (AA 36-85)

Catalog No. ABIN6744440

Product Details anti-MKNK2 Antibody

Target: MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MKNK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human MKNK2 / MNK2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig, Zebrafish (100%) Bovine (92%) Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human MKNK2 (Q9HBH9, NP_951009). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Zebra finch, Stickleback, Zebrafish (100%), Bovine (92%), Xenopus (91%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MKNK2

Target: MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))

Alternative Name: MKNK2 / MNK2 (MKNK2 Products)

Synonyms: GPRK7 antibody, MNK2 antibody, gprk7 antibody, mnk2 antibody, 2010016G11Rik antibody, Gprk7 antibody, Mnk2 antibody, mknk2 antibody, wu:fb37e05 antibody, wz5090 antibody, fi34c11 antibody, wu:fi34c11 antibody, wu:fi41e12 antibody, zgc:55587 antibody, MAP kinase interacting serine/threonine kinase 2 antibody, MAP kinase-interacting serine/threonine kinase 2 antibody, MAP kinase interacting serine/threonine kinase 2b antibody, MAP kinase interacting serine/threonine kinase 2 L homeolog antibody, MAP kinase interacting serine/threonine kinase 2a antibody, MKNK2 antibody, mknk2 antibody, Mknk2 antibody, mknk2b antibody, mknk2.L antibody, mknk2a antibody

Background: Name/Gene ID: MKNK2
Subfamily: MNK
Family: Protein Kinase

Synonyms: MKNK2, GPRK7, MNK2, MAPK-Interacting Kinase 2

Gene ID: 2872

NCBI Accession: NP_951009

UniProt: Q9HBH9

Pathways: MAPK Signaling