XIAP antibody (AA 201-330)

Catalog No. ABIN674364

Product Details anti-XIAP Antibody

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Binding Specificity: AA 201-330

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XIAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse XIAP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-XIAP antibody (ABIN674364)

Sun, Liu, Zou, Lan, Sun, Wang, Zhao, Jiang, Liu: "Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer." in: Journal of bioenergetics and biomembranes, (2015) (PubMed).

Jin, Lan, Han, Sun, Liu, Zhang, Liu, Zhang, Hu, Liu, Wang: "Smac mimetic?induced caspase?independent necroptosis requires RIP1 in breast cancer." in: Molecular medicine reports, (2015) (PubMed).

Chen, Xie, Zhao, Wang, Bai, Yin, Jiang, Xie, Jia, Huang et al.: "Ampelopsin induces apoptosis by regulating multiple c?Myc/S?phase kinase?associated protein 2/F?box and WD repeat?containing protein 7/histone deacetylase 2 pathways in human lung adenocarcinoma ..." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 105-12, (2014) (PubMed).

Chen, Bai, Zhong, Xie, Long, Yang, Wu, Jia, Wang: "Wogonin has multiple anti-cancer effects by regulating c-Myc/SKP2/Fbw7? and HDAC1/HDAC2 pathways and inducing apoptosis in human lung adenocarcinoma cell line A549." in: PLoS ONE, Vol. 8, Issue 11, pp. e79201, (2013) (PubMed).

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: XIAP/BIRC4 (XIAP Products)

Synonyms: API3 antibody, BIRC4 antibody, IAP-3 antibody, ILP1 antibody, MIHA antibody, XLP2 antibody, hIAP-3 antibody, hIAP3 antibody, api3 antibody, birc4 antibody, ilp1 antibody, miha antibody, xlp2 antibody, xxiap antibody, 1110015C02Rik antibody, Aipa antibody, Api3 antibody, Birc4 antibody, IAP3 antibody, ILP-1 antibody, mXIAP antibody, riap3 antibody, fb73d01 antibody, wu:fb73d01 antibody, zgc:91873 antibody, BIRC8 antibody, X-linked inhibitor of apoptosis antibody, X-linked inhibitor of apoptosis L homeolog antibody, XIAP antibody, xiap antibody, xiap.L antibody, Xiap antibody

Background:

Synonyms: Aipa, Api3, IAP3, MIHA, Birc4, ILP-1, 11115C2Rik, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, IAP homolog A, Inhibitor of apoptosis protein 3, IAP-3, mIAP-3, mIAP3, X-linked inhibitor of apoptosis protein, X-linked IAP, Xiap

Background: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.

Gene ID: 11798

UniProt: Q60989

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis