8-OHDG antibody

Catalog No. ABIN674319

Product Details anti-8-OHDG Antibody

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 8-OHDG antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): 8-OHdG

Purification: Purified by Protein A.

Immunogen: KLH conjugated 8-OhdG

Isotype: IgG

Application Details

Application Notes: IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-8-OHDG antibody (ABIN674319)

Leem, Park, Park, Kim, Kim: "Suppression of neuroinflammation and α-synuclein oligomerization by rotarod walking exercise in subacute MPTP model of Parkinson's disease." in: Neurochemistry international, Vol. 165, pp. 105519, (2023) (PubMed).

Du, West, Cai, Cheng, Ewert, Li, Floyd, Kopke: "Antioxidants reduce neurodegeneration and accumulation of pathologic Tau proteins in the auditory system after blast exposure." in: Free radical biology & medicine, Vol. 108, pp. 627-643, (2017) (PubMed).

Yu, Bei, Li, Han, Zhong, Liu, Zhao, Zhu, Zhao: "In vitro the differences of inflammatory and oxidative reactions due to sulfur mustard induced acute pulmonary injury underlying intraperitoneal injection and intratracheal instillation in rats." in: International immunopharmacology, Vol. 47, pp. 78-87, (2017) (PubMed).

Narayanaswamy, Baral, Haller, Dumler, Acharya, Kiyan: "Transcriptomic pathway analysis of urokinase receptor silenced breast cancer cells: a microarray study." in: Oncotarget, Vol. 8, Issue 60, pp. 101572-101590, (2017) (PubMed).

Tumurkhuu, Shimada, Dagvadorj, Crother, Zhang, Luthringer, Gottlieb, Chen, Arditi: "Ogg1-Dependent DNA Repair Regulates NLRP3 Inflammasome and Prevents Atherosclerosis." in: Circulation research, Vol. 119, Issue 6, pp. e76-90, (2016) (PubMed).

Stangenberg, Nguyen, Chen, Al-Odat, Killingsworth, Gosnell, Anwer, Goldys, Pollock, Saad: "Oxidative stress, mitochondrial perturbations and fetal programming of renal disease induced by maternal smoking." in: The international journal of biochemistry & cell biology, Vol. 64, pp. 81-90, (2015) (PubMed).

Feng, Huang, Yang, Yan, Jin, Cheng, Cui: "Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats' testis." in: Mutation research. Genetic toxicology and environmental mutagenesis, Vol. 792, pp. 35-45, (2015) (PubMed).

Zhang, Zhao, Fan, Chen, Quan: "Electrochemiluminescence immunosensor for highly sensitive detection of 8-hydroxy-2'-deoxyguanosine based on carbon quantum dot coated Au/SiO2 core-shell nanoparticles." in: Talanta, Vol. 131, pp. 379-85, (2014) (PubMed).

Yong, Chen, Shen, Vijayaraj, Ma, Pollock, Saad: "Plumbagin ameliorates diabetic nephropathy via interruption of pathways that include NOX4 signalling." in: PLoS ONE, Vol. 8, Issue 8, pp. e73428, (2013) (PubMed).

Target Details for 8-OHDG

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Abstract: 8-OHDG Products

Target Type: Chemical

Background:

Synonyms: 8-Hydroxy-2'-deoxyguanosine, 8-Hydroxydeoxyguanosine, 8 hydroxyguanine, 8 hydroxyguanosine, 8 OHG, 8-OHG, 8OG, 8OHdG, 8OHG,

Background: 8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease.