PIWIL2 antibody (AA 287-336)

Catalog No. ABIN6742057

Product Details anti-PIWIL2 Antibody

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Binding Specificity: AA 287-336

Reactivity: Human, Cow, Dog, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PIWIL2

Predicted Reactivity: Percent identity by BLAST analysis: Bovine, Pig (100%) Rat, Horse, Guinea pig (92%) Dog (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa287-336 of human PIWIL2 (Q8TC59, NP_060538). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Bovine, Pig (100%), Galago, Mouse, Rat, Bat, Rabbit, Horse, Guinea pig (92%), Marmoset, Opossum (85%), Dog (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PIWIL2

Target: PIWIL2 (Piwi-Like 2 (PIWIL2))

Alternative Name: PIWIL2 (PIWIL2 Products)

Synonyms: si:dkey-88f5.1 antibody, zili antibody, Piwil1l antibody, mili antibody, CT80 antibody, HILI antibody, PIWIL1L antibody, piwil2 antibody, piwi like RNA-mediated gene silencing 2 antibody, piwi-like RNA-mediated gene silencing 2 antibody, piwil2 antibody, Piwil2 antibody, PIWIL2 antibody

Background: Name/Gene ID: PIWIL2

Synonyms: PIWIL2, Cancer/testis antigen 80, CT80, HILI, Mili, Piwi-like 2 (Drosophila), PIWIL1L, Miwi like, Piwi-like protein 2, Piwil2-like protein

Gene ID: 55124

NCBI Accession: NP_060538

UniProt: Q8TC59

Pathways: Stem Cell Maintenance