GEM antibody (AA 35-84)

Catalog No. ABIN6741918

Product Details anti-GEM Antibody

Target: GEM (GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))

Binding Specificity: AA 35-84

Reactivity: Human, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Monkey, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GEM antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human GEM

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Pig (100%) Rat (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human GEM (P55040, NP_005252). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Rat, Bat, Platypus, Xenopus (92%), Lizard (85%), Zebra finch (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GEM

Target: GEM (GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))

Alternative Name: GEM / KIR (GEM Products)

Synonyms: KIR antibody, GEM antibody, DKFZp470D0612 antibody, LOC100221129 antibody, kir antibody, xgem antibody, zgc:153003 antibody, Gem antibody, AV020497 antibody, GTP binding protein overexpressed in skeletal muscle antibody, geminin, DNA replication inhibitor antibody, GTP binding protein (gene overexpressed in skeletal muscle) antibody, GEM antibody, gem antibody, Gem antibody, GMNN antibody

Background: Name/Gene ID: GEM
Family: Ras GTPase superfamily IPR001806

Synonyms: GEM, GTP-binding protein GEM, KIR, RAS-like protein KIR

Gene ID: 2669

NCBI Accession: NP_005252

UniProt: P55040