EED antibody (AA 35-84)

Catalog No. ABIN6741861

Product Details anti-EED Antibody

Target: EED (Embryonic Ectoderm Development (EED))

Binding Specificity: AA 35-84

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human EED

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig, Guinea pig, Chicken (100%) Rabbit, Xenopus (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human EED (O75530, NP_003788). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard (100%), Rabbit, Xenopus, Medaka (92%), Platypus (90%), Opossum, Stickleback (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:2500.

Not recommended for: IF, IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED (EED Products)

Synonyms: Xeed antibody, heed antibody, wait1 antibody, EED antibody, HEED antibody, WAIT1 antibody, zgc:112509 antibody, Xeed-B antibody, eed antibody, psXEED antibody, ENSMUSG00000039373 antibody, l(7)5Rn antibody, l7Rn5 antibody, lusk antibody, Xeed-A antibody, embryonic ectoderm development antibody, embryonic ectoderm development S homeolog antibody, microRNA 6755 antibody, embryonic ectoderm development L homeolog antibody, eed antibody, EED antibody, eed.S antibody, MIR6755 antibody, Eed antibody, eed.L antibody

Background: Name/Gene ID: EED

Synonyms: EED, Embryonic ectoderm development, HEED, WAIT1, WAIT-1, Polycomb protein EED

Gene ID: 8726

NCBI Accession: NP_003788

UniProt: O75530