AGO1 antibody (N-Term)

Catalog No. ABIN6741837

Product Details anti-AGO1 Antibody

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Monkey, Cow, Horse, Rabbit, Guinea Pig, Pig, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human EIF2C1 / AGO1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human AGO1 (Q9UL18, NP_036331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Platypus, Xenopus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: AGO1 / EIF2C (EIF2C1 Products)

Background: Name/Gene ID: AGO1

Synonyms: AGO1, Argonaute 1, EIF-2C 1, EIF2C1, GERP95, EIF2C, EIF2C 1, HAgo1, Q99, Argonaute1, Protein argonaute-1

Gene ID: 26523

NCBI Accession: NP_036331

UniProt: Q9UL18

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization