Klotho antibody (AA 467-516)

Catalog No. ABIN6741741

Product Details anti-Klotho Antibody

Target: Klotho (KL)

Binding Specificity: AA 467-516

Reactivity: Human, Rabbit, Horse, Dog, Pig, Bat, Monkey, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Klotho antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human KL / Klotho

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Pig, Chicken (100%) Mouse, Rat, Bovine, Guinea pig, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa467-516 of human KL (Q9UEF7, NP_004786). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bat, Rabbit, Horse, Pig, Turkey, Zebra finch, Chicken, Platypus (100%), Mouse, Rat, Bovine, Guinea pig, Zebrafish (92%), Opossum (85%), Xenopus (84%), Stickleback (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Klotho

Target: Klotho (KL)

Alternative Name: KL / Klotho (KL Products)

Synonyms: alpha-kl antibody, KL antibody, LOC100223456 antibody, klotho antibody, Klotho antibody, KL antibody, Kl antibody, kl antibody, LOC100533542 antibody

Background: Name/Gene ID: KL

Synonyms: KL, Klotho

Gene ID: 9365

NCBI Accession: NP_004786

UniProt: Q9UEF7

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding