POLB antibody (AA 107-156)

Catalog No. ABIN6741696

Product Details anti-POLB Antibody

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Binding Specificity: AA 107-156

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Hamster, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human POLB / DNA Polymerase Beta

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Rabbit, Horse, Guinea pig (100%) Pig (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human POLB (P06746, NP_002681). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Elephant, Pig, Opossum (92%), Turkey, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB / DNA Polymerase Beta (POLB Products)

Synonyms: wu:fa97a05 antibody, zgc:109924 antibody, POLB antibody, DDBDRAFT_0186152 antibody, DDBDRAFT_0215784 antibody, DDBDRAFT_0232376 antibody, DDB_0186152 antibody, DDB_0215784 antibody, DDB_0232376 antibody, A430088C08Rik antibody, polymerase (DNA directed), beta antibody, DNA polymerase beta antibody, DNA polymerase X family protein antibody, polymerase (DNA directed), beta S homeolog antibody, polb antibody, POLB antibody, polB antibody, polb.S antibody, Polb antibody

Background: Name/Gene ID: POLB

Synonyms: POLB, DNA pol beta, Mutant DNA polymerase beta, DNA polymerase beta, DNA polymerase beta subunit

Gene ID: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: DNA Damage Repair