Pleckstrin antibody (N-Term)
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- Target See all Pleckstrin (PLEK) Antibodies
- Pleckstrin (PLEK)
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Binding Specificity
- N-Term
- Reactivity
- Human, Mouse, Rat, Cow, Guinea Pig, Zebrafish (Danio rerio), Bat, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Pleckstrin antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Human PLEK
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig, Zebrafish (100%) Dog, Horse, Chicken (92%) Xenopus (83%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from N-Terminus of human PLEK (P08567, NP_002655). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Bovine, Bat, Pig, Guinea pig, Lizard, Zebrafish (100%), Monkey, Elephant, Dog, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus, Trout, Salmon, Stickleback (84%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product PLEK Primary Antibody
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- Application Notes
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Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- Pleckstrin (PLEK)
- Alternative Name
- PLEK / Pleckstrin (PLEK Products)
- Synonyms
- MGC69065 antibody, PLEK antibody, P47 antibody, 2010300B13Rik antibody, pleckstrin L homeolog antibody, pleckstrin antibody, plek.L antibody, PLEK antibody, Plek antibody
- Background
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Name/Gene ID: PLEK
Synonyms: PLEK, Platelet 47 kDa protein, Pleckstrin, p47 - Gene ID
- 5341
- NCBI Accession
- NP_002655
- UniProt
- P08567
- Pathways
- Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process
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