RNF14 antibody (N-Term)

Catalog No. ABIN6741638

Product Details anti-RNF14 Antibody

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF14 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human RNF14

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse, Pig, Guinea pig, Chicken (100%) Rabbit (92%) Mouse, Rat, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human RNF14 (Q9UBS8, NP_004281). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Elephant, Dog, Bovine, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus (100%), Orangutan, Marmoset, Bat, Rabbit, Stickleback (92%), Xenopus (91%), Mouse, Rat, Lizard (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RNF14

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Alternative Name: RNF14 / ARA54 (RNF14 Products)

Synonyms: ARA54 antibody, HFB30 antibody, TRIAD2 antibody, 2310075C09Rik antibody, 2610005D23Rik antibody, AA986456 antibody, AU041447 antibody, D18Ertd188e antibody, D7Bwg0165e antibody, Triad2 antibody, ring finger protein 14 antibody, RNF14 antibody, Rnf14 antibody

Background: Name/Gene ID: RNF14

Synonyms: RNF14, HRIHFB2038, HFB30, Triad2 protein, ARA54, Ring finger protein 14, TRIAD2

Gene ID: 9604

NCBI Accession: NP_004281

UniProt: Q9UBS8

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling