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ZNF777 antibody (AA 468-517)

ZNF777 Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741624
  • Target See all ZNF777 Antibodies
    ZNF777 (Zinc Finger Protein 777 (ZNF777))
    Binding Specificity
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 468-517
    Reactivity
    • 10
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig
    Host
    • 8
    • 2
    Rabbit
    Clonality
    • 10
    Polyclonal
    Conjugate
    • 10
    This ZNF777 antibody is un-conjugated
    Application
    • 10
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human ZNF777
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa468-517 of human ZNF777 (Q9ULD5, NP_056509). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Gorilla, Marmoset, Dog, Bat (92%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product ZNF777 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    ZNF777 (Zinc Finger Protein 777 (ZNF777))
    Alternative Name
    ZNF777 (ZNF777 Products)
    Synonyms
    2500002G23Rik antibody, ZNF777 antibody, RGD1566056 antibody, Znf777 antibody, zinc finger protein 777 antibody, Zfp777 antibody, ZNF777 antibody, LOC769049 antibody
    Background
    Name/Gene ID: ZNF777
    Family: Zinc Finger

    Synonyms: ZNF777, Zinc finger protein 777, KIAA1285
    Gene ID
    27153
    NCBI Accession
    NP_056509
    UniProt
    Q9ULD5
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