ARID5A antibody (C-Term)

Catalog No. ABIN6741610

Product Details anti-ARID5A Antibody

Target: ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ARID5A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ARID5A

Target: ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))

Alternative Name: MRF-1 / ARID5A (ARID5A Products)

Synonyms: MRF-1 antibody, MRF1 antibody, RP11-363D14 antibody, D430024K22Rik antibody, Mrf1 antibody, AT-rich interaction domain 5A antibody, AT rich interactive domain 5A (MRF1-like) antibody, ARID5A antibody, Arid5a antibody, arid5a antibody

Background: Name/Gene ID: ARID5A

Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I

Gene ID: 10865

NCBI Accession: NP_997646

UniProt: Q03989