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YAP1 antibody (AA 351-400)

YAP1 Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Monkey, Dog, Sheep, Chicken, Cow, Horse, Pig, Xenopus laevis, Rabbit, Bat WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN462247
  • Target See all YAP1 Antibodies
    YAP1 (Yes-Associated Protein 1 (YAP1))
    Binding Specificity
    • 25
    • 15
    • 15
    • 14
    • 10
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 351-400
    Reactivity
    Human, Mouse, Rat, Zebrafish (Danio rerio), Monkey, Dog, Sheep, Chicken, Cow, Horse, Pig, Xenopus laevis, Rabbit, Bat
    Host
    • 129
    • 17
    • 1
    • 1
    Rabbit
    Clonality
    • 120
    • 27
    • 1
    Polyclonal
    Conjugate
    • 78
    • 9
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This YAP1 antibody is un-conjugated
    Application
    • 107
    • 53
    • 30
    • 27
    • 27
    • 25
    • 21
    • 18
    • 12
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human YAP1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Chicken, Xenopus, Zebrafish (100%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa351-400 of human YAP1 (P46937, NP_001123617). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product YAP1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled Water.
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    YAP1 (Yes-Associated Protein 1 (YAP1))
    Alternative Name
    YAP / YAP1 (YAP1 Products)
    Synonyms
    YAP antibody, YAP2 antibody, YAP65 antibody, YKI antibody, xyap antibody, yap antibody, yap2 antibody, yap65 antibody, yki antibody, AI325207 antibody, Yap antibody, Yap65 antibody, Yki antibody, Yorkie antibody, cb194 antibody, sb:cb194 antibody, si:ch211-181p1.5 antibody, si:dkey-3b8.3 antibody, wu:fc18c04 antibody, zgc:158380 antibody, Yes associated protein 1 antibody, Yes associated protein 1 S homeolog antibody, yes-associated protein 1 antibody, Yes-associated protein 1 antibody, YAP1 antibody, yap1.S antibody, Yap1 antibody, yap1 antibody
    Background
    Name/Gene ID: YAP1

    Synonyms: YAP1, 65 kDa Yes-associated protein, YAP, Yes-associated protein beta, Yes-associated protein delta, Yorkie homolog, YAP2, Yes-associated protein 1, Yes-associated protein 2, YKI, YAP65
    Gene ID
    10413
    NCBI Accession
    NP_001123617
    UniProt
    P46937
    Pathways
    MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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