GTPBP10 antibody (AA 254-303)

Catalog No. ABIN6741598

Product Details anti-GTPBP10 Antibody

Target: GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

Binding Specificity: AA 254-303

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTPBP10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GTPBP10

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig (100%) Dog, Guinea pig (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa254-303 of human GTPBP10 (A4D1E9, NP_149098). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Rabbit, Horse, Pig, Opossum, Armadillo, Lizard, Stickleback (100%), Dog, Bovine, Bat, Guinea pig (92%), Salmon (90%), Turkey, Zebra finch, Chicken, Platypus (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GTPBP10

Target: GTPBP10 (GTP-Binding Protein 10 (GTPBP10))

Alternative Name: GTPBP10 (GTPBP10 Products)

Synonyms: zgc:92334 antibody, ObgH2 antibody, 4930545J22Rik antibody, BC034507 antibody, Cldn12 antibody, GTP-binding protein 10 (putative) antibody, GTP binding protein 10 antibody, gtpbp10 antibody, GTPBP10 antibody, Gtpbp10 antibody

Background: Name/Gene ID: GTPBP10

Synonyms: GTPBP10, DKFZP686A10121, ObgH2, Protein obg homolog 2, UG0751c10, GTP-binding protein 10

Gene ID: 85865

NCBI Accession: NP_149098

UniProt: A4D1E9