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PNPT1 antibody (AA 489-538)

PNPT1 Reactivity: Human, Rat, Dog, Horse, Cow, Guinea Pig, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741592
  • Target See all PNPT1 Antibodies
    PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
    Binding Specificity
    • 15
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 489-538
    Reactivity
    • 41
    • 22
    • 18
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Dog, Horse, Cow, Guinea Pig, Pig
    Host
    • 55
    • 3
    Rabbit
    Clonality
    • 57
    • 1
    Polyclonal
    Conjugate
    • 26
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PNPT1 antibody is un-conjugated
    Application
    • 49
    • 21
    • 13
    • 13
    • 10
    • 9
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human PNPT1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human (100%) Mouse (92%) Rat, Guinea pig (85%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa489-538 of human PNPT1 (Q8TCS8, NP_149100). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse (92%), Rat, Guinea pig (85%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product PNPT1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 0.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute with 50 μL sterile ddH2O.
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))
    Alternative Name
    PNPT1 (PNPT1 Products)
    Synonyms
    COXPD13 antibody, DFNB70 antibody, OLD35 antibody, PNPASE antibody, old-35 antibody, 1200003F12Rik antibody, Old35 antibody, PNPase antibody, Pnptl1 antibody, polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody, polyribonucleotide nucleotidyltransferase 1 antibody, CpipJ_CPIJ005886 antibody, PNPT1 antibody, Pnpt1 antibody
    Background
    Name/Gene ID: PNPT1

    Synonyms: PNPT1, 3-5 RNA exonuclease OLD35, COXPD13, DFNB70, OLD35, PNPase 1, PNPase old-35, Old-35, PNPASE, Polynucleotide phosphorylase 1
    Gene ID
    87178
    NCBI Accession
    NP_149100
    UniProt
    Q8TCS8
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