RPF1 antibody (AA 45-94)

Catalog No. ABIN6741590

Product Details anti-RPF1 Antibody

Target: RPF1 (Brix Domain Containing 5 (RPF1))

Binding Specificity: AA 45-94

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RPF1 / BXDC5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine (100%) Dog, Armadillo (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa45-94 of human RPF1 (Q9H9Y2, NP_079341). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig (100%), Dog, Zebra finch, Chicken, Armadillo (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RPF1

Target: RPF1 (Brix Domain Containing 5 (RPF1))

Alternative Name: BXDC5 / RPF1 (RPF1 Products)

Background: Name/Gene ID: RPF1

Synonyms: RPF1, BBR140, Brix domain containing 5, BXDC5, Ribosome production factor 1, RNA processing factor 1 (RPF1), RP11-118B23.1

Gene ID: 80135

NCBI Accession: NP_079341

UniProt: Q9H9Y2