SSB antibody (AA 82-131)

Catalog No. ABIN6741588

Product Details anti-SSB Antibody

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Binding Specificity: AA 82-131

Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse, Rabbit, Cow, Guinea Pig, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SSB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse (100%) Pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa82-131 of human SSB (P05455, NP_003133). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Pig, Opossum, Platypus (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SSB

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Alternative Name: SSB / La (SSB Products)

Background: Name/Gene ID: SSB

Synonyms: SSB, Autoantigen La, La, La ribonucleoprotein, Lupus La antigen, SS-B, SS-B/La protein, La autoantigen, LARP3, Lupus La protein

Gene ID: 6741

NCBI Accession: NP_003133

UniProt: P05455