TRIM8 antibody (AA 36-85)
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- Target See all TRIM8 Antibodies
- TRIM8 (Tripartite Motif Containing 8 (TRIM8))
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Binding Specificity
- AA 36-85
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Reactivity
- Human, Mouse, Rat, Dog, Cow, Rabbit, Pig, Zebrafish (Danio rerio), Hamster, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TRIM8 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human TRIM8 / GERP
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product TRIM8 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- TRIM8 (Tripartite Motif Containing 8 (TRIM8))
- Alternative Name
- TRIM8 / GERP (TRIM8 Products)
- Synonyms
- GERP antibody, RNF27 antibody, AA408830 antibody, BB006755 antibody, Rnf27 antibody, si:ch211-208d15.5 antibody, wu:fc90g07 antibody, tripartite motif containing 8 antibody, tripartite motif containing 8 L homeolog antibody, tripartite motif-containing 8 antibody, tripartite motif containing 8b antibody, TRIM8 antibody, trim8.L antibody, Trim8 antibody, trim8b antibody
- Background
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Name/Gene ID: TRIM8
Family: Tripartite Motif
Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8 - Gene ID
- 81603
- NCBI Accession
- NP_112174
- UniProt
- Q9BZR9
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