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TRIM8 antibody (AA 36-85)

TRIM8 Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Pig, Zebrafish (Danio rerio), Hamster, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6741584
  • Target See all TRIM8 Antibodies
    TRIM8 (Tripartite Motif Containing 8 (TRIM8))
    Binding Specificity
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 36-85
    Reactivity
    • 35
    • 27
    • 20
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog, Cow, Rabbit, Pig, Zebrafish (Danio rerio), Hamster, Monkey
    Host
    • 32
    • 2
    • 1
    Rabbit
    Clonality
    • 35
    Polyclonal
    Conjugate
    • 10
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TRIM8 antibody is un-conjugated
    Application
    • 23
    • 13
    • 7
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human TRIM8 / GERP
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Horse, Chicken, Xenopus (92%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa36-85 of human TRIM8 (Q9BZR9, NP_112174). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (100%), Horse, Turkey, Zebra finch, Chicken, Lizard, Xenopus (92%), Opossum (91%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product TRIM8 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    TRIM8 (Tripartite Motif Containing 8 (TRIM8))
    Alternative Name
    TRIM8 / GERP (TRIM8 Products)
    Synonyms
    GERP antibody, RNF27 antibody, AA408830 antibody, BB006755 antibody, Rnf27 antibody, si:ch211-208d15.5 antibody, wu:fc90g07 antibody, tripartite motif containing 8 antibody, tripartite motif containing 8 L homeolog antibody, tripartite motif-containing 8 antibody, tripartite motif containing 8b antibody, TRIM8 antibody, trim8.L antibody, Trim8 antibody, trim8b antibody
    Background
    Name/Gene ID: TRIM8
    Family: Tripartite Motif

    Synonyms: TRIM8, RING finger protein 27, Tripartite motif-containing 8, Tripartite motif containing 8, GERP, RNF27, Tripartite motif protein TRIM8
    Gene ID
    81603
    NCBI Accession
    NP_112174
    UniProt
    Q9BZR9
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