SOX17 antibody (C-Term)

Catalog No. ABIN6741583

Product Details anti-SOX17 Antibody

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SOX17

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Guinea pig (92%) Mouse, Rat, Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SOX17 (Q9H6I2, NP_071899). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Elephant, Panda, Bat, Pig, Guinea pig (92%), Galago, Marmoset, Mouse, Rat, Dog (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SOX17

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Alternative Name: SOX17 (SOX17 Products)

Synonyms: VUR3 antibody, SOX17 antibody, Sox17alpha antibody, Xsox17 antibody, Xsox17-alpha antibody, Xsox17alpha antibody, sox17-alpha antibody, tSox17alpha antibody, xSox17alpha1 antibody, xSox17alpha2 antibody, xsox17a antibody, sox17 antibody, sox7/17 antibody, Sox antibody, SRY-box 17 antibody, SRY (sex determining region Y)-box 17 antibody, SRY box 17 antibody, SRY-box 17 alpha antibody, sox7/17 protein antibody, SOX17 antibody, sox17 antibody, Sox17 antibody, sox17a antibody, sox7/17 antibody

Background: Name/Gene ID: SOX17
Family: Transcription factor

Synonyms: SOX17, Transcription factor SOX-17, VUR3

Gene ID: 64321

NCBI Accession: NP_071899

UniProt: Q9H6I2