TRIM68 antibody (AA 395-444)

Catalog No. ABIN6741579

Product Details anti-TRIM68 Antibody

Target: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Binding Specificity: AA 395-444

Reactivity: Human, Mouse, Cow, Horse, Pig, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM68 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TRIM68

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Horse, Pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa395-444 of human TRIM68 (Q6AZZ1, NP_060543). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Horse, Pig (100%), Galago, Bat (92%), Opossum (84%), Turkey, Chicken (80%), Rat (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile dH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM68

Target: TRIM68 (Tripartite Motif Containing 68 (TRIM68))

Alternative Name: TRIM68 (TRIM68 Products)

Synonyms: GC109 antibody, RNF137 antibody, SS-56 antibody, SS56 antibody, AI894158 antibody, F730114J12Rik antibody, Rnf137 antibody, TRIM68 antibody, tripartite motif containing 68 antibody, tripartite motif-containing 68 antibody, E3 ubiquitin-protein ligase TRIM68 antibody, TRIM68 antibody, Trim68 antibody, LOC100053519 antibody

Background: Name/Gene ID: TRIM68
Family: Tripartite Motif

Synonyms: TRIM68, GC109, RNF137, Ro/SSA1 related protein, SS-56, Tripartite motif containing 68, RING finger protein 137, SS56, Tripartite motif-containing 68, SSA protein SS-56

Gene ID: 55128

NCBI Accession: NP_060543

UniProt: Q6AZZ1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling