NKRF antibody (C-Term)

Catalog No. ABIN6741576

Product Details anti-NKRF Antibody

Target: NKRF (NFKB Repressing Factor (NKRF))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig, Pig, Monkey, Bat, Hamster, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NKRF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NKRF

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human NKRF (O15226, NP_060014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NKRF

Target: NKRF (NFKB Repressing Factor (NKRF))

Alternative Name: NKRF / NRF (NKRF Products)

Synonyms: NKRF antibody, NRF antibody, nrf antibody, itba4 antibody, 9430034D17Rik antibody, AW124770 antibody, C80287 antibody, ITBA4 antibody, RGD1563950 antibody, NFKB repressing factor antibody, NFKB repressing factor L homeolog antibody, NF-kappaB repressing factor antibody, NFKB repressing factor-like antibody, NKRF antibody, nkrf antibody, nkrf.L antibody, Nkrf antibody, Nkrfl antibody

Background: Name/Gene ID: NKRF

Synonyms: NKRF, NFkB-repressing factor, ITBA4, Protein ITBA4, NF-kappaB repressing factor, NFKB repressing factor, Transcription factor NRF, NF-kappa-B-repressing factor, NRF

Gene ID: 55922

NCBI Accession: NP_060014

UniProt: O15226