LDOC1 antibody (N-Term)

Catalog No. ABIN462213

Product Details anti-LDOC1 Antibody

Target: LDOC1 (Leucine Zipper, Down-Regulated in Cancer 1 (LDOC1))

Binding Specificity: N-Term

Reactivity: Human, Cow, Rabbit, Dog, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDOC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human LDOC1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Rabbit, Pig, Guinea pig (100%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LDOC1 (O95751, NP_036449). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Panda, Bovine, Rabbit, Pig, Guinea pig (100%), Monkey, Bat, Horse (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for LDOC1

Target: LDOC1 (Leucine Zipper, Down-Regulated in Cancer 1 (LDOC1))

Alternative Name: LDOC1 (LDOC1 Products)

Synonyms: BCUR1 antibody, Mar7 antibody, Mart7 antibody, RGD1565644 antibody, Gm366 antibody, LDOC1 antibody, LDOC1, regulator of NFKB signaling antibody, regulator of NFKB signaling antibody, LDOC1 antibody, Ldoc1 antibody

Background: Name/Gene ID: LDOC1

Synonyms: LDOC1, Breast cancer, up-regulated 1, Mart7, BCUR1, Mar7, Protein LDOC1

Gene ID: 23641

NCBI Accession: NP_036449

UniProt: O95751