NR2C1 antibody (N-Term)
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- Target See all NR2C1 Antibodies
- NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Dog, Horse, Rabbit, Pig, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NR2C1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NR2C1 / TR2-11
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig (100%) Bovine, Guinea pig (92%) Chicken (84%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from N-Terminus of human NR2C1 (Q15626, NP_001027458). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Pig (100%), Hamster, Bovine, Opossum, Guinea pig, Platypus, Lizard, Zebrafish (92%), Bat, Turkey, Zebra finch, Chicken, Xenopus (84%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- NR2C1 (Nuclear Receptor Subfamily 2, Group C, Member 1 (NR2C1))
- Alternative Name
- NR2C1 (NR2C1 Products)
- Synonyms
- TR2 antibody, NR2C1 antibody, dor2 antibody, tr2-11 antibody, xDOR2 antibody, DKFZp468M0512 antibody, si:dkey-183c16.2 antibody, zgc:110672 antibody, 4831444H07Rik antibody, 80.3 antibody, Eenr antibody, Tr2-11 antibody, Tr2 antibody, Psg4 antibody, TR2-11 antibody, PSG9 antibody, nr2c1 antibody, nuclear receptor subfamily 2 group C member 1 antibody, nuclear receptor subfamily 2 group C member 1 S homeolog antibody, nuclear receptor subfamily 2, group C, member 1 antibody, pregnancy specific beta-1-glycoprotein 4 antibody, nuclear receptor subfamily 2 group C member 1 L homeolog antibody, NR2C1 antibody, nr2c1.S antibody, nr2c1 antibody, Nr2c1 antibody, PSG4 antibody, nr2c1.L antibody
- Background
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Name/Gene ID: NR2C1
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR
Synonyms: NR2C1, Orphan nuclear receptor TR2, Testicular orphan receptor-2, TR2, TR2 nuclear hormone receptor, TR2-11, Testicular receptor 2 - Gene ID
- 7181
- NCBI Accession
- NP_001027458
- UniProt
- P13056
- Pathways
- Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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