SP4 antibody (C-Term)

Catalog No. ABIN6741568

Product Details anti-SP4 Antibody

Target: SP4 (Sp4 Transcription Factor (SP4))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Horse, Rabbit, Cow, Dog, Monkey, Pig, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SP4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SP4 (Q02446, NP_003103). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Lizard, Xenopus (100%), Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SP4

Target: SP4 (Sp4 Transcription Factor (SP4))

Alternative Name: SP4 (SP4 Products)

Synonyms: zgc:64226 antibody, DKFZp469M0517 antibody, HF1B antibody, SPR-1 antibody, HF-1b antibody, 5730497N03Rik antibody, HF1-b antibody, sp4 transcription factor antibody, Sp4 transcription factor antibody, Sp4 transcription factor L homeolog antibody, trans-acting transcription factor 4 antibody, sp4 antibody, SP4 antibody, sp4.L antibody, Sp4 antibody

Background: Name/Gene ID: SP4

Synonyms: SP4, HF1B, Sp4 transcription factor, SPR-1, Transcription factor Sp4

Gene ID: 6671

NCBI Accession: NP_003103

UniProt: Q02446