RHOXF2 antibody (AA 108-157)

Catalog No. ABIN6741566

Product Details anti-RHOXF2 Antibody

Target: RHOXF2 (Rhox Homeobox Family, Member 2 (RHOXF2))

Binding Specificity: AA 108-157

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOXF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RHOXF2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human RHOXF2 (Q9BQY4, NP_115887). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%), Monkey (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RHOXF2

Target: RHOXF2 (Rhox Homeobox Family, Member 2 (RHOXF2))

Alternative Name: RHOXF2 (RHOXF2 Products)

Synonyms: CT107 antibody, PEPP-2 antibody, PEPP2 antibody, THG1 antibody, Rhox homeobox family member 2 antibody, RHOXF2 antibody

Background: Name/Gene ID: RHOXF2

Synonyms: RHOXF2, Cancer/testis antigen 107, Homeobox protein from AL590526, PEPP subfamily gene 2, PEPP2, PEPP-2, Rhox homeobox family, member 2, Rhox homeobox family member 2, THG1, Testis homeobox gene 1, CT107

Gene ID: 84528

NCBI Accession: NP_115887

UniProt: Q9BQY4