SSX4 antibody (AA 108-157)

Catalog No. ABIN462200

Product Details anti-SSX4 Antibody

Target: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Binding Specificity: AA 108-157

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SSX4

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human SSX4 (O60224, NP_005627). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey (85%), Gibbon (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SSX4

Target: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Alternative Name: SSX4 (SSX4 Products)

Synonyms: CT5.4 antibody, SSX family member 4 antibody, SSX4 antibody

Background: Name/Gene ID: SSX4

Synonyms: SSX4, CT5.4, Cancer/testis antigen 5.4, Protein SSX4, SSX4A, SSX4B

Gene ID: 6759

NCBI Accession: NP_005627