NRF1 antibody (AA 360-409)
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- Target See all NRF1 Antibodies
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
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Binding Specificity
- AA 360-409
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Reactivity
- Human, Rat, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio), Dog, Monkey, Chicken, Pig, Xenopus laevis
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NRF1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NRF1
- No Cross-Reactivity
- Mouse (Murine)
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product NRF1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
- Alternative Name
- NRF1 / NRF-1 (NRF1 Products)
- Synonyms
- ALPHA-PAL antibody, C87038 antibody, D6Ertd415e antibody, SC:BZ3C13.1 antibody, SC:bZ3C13.2 antibody, ube2h antibody, Nfe2l1_retired antibody, IBR/F antibody, NRF1 antibody, alpha-pal antibody, nrf-1 antibody, LOC100220087 antibody, nuclear respiratory factor 1 antibody, nuclear respiratory factor 1 S homeolog antibody, NRF1 antibody, Nrf1 antibody, nrf1 antibody, nrf1.S antibody
- Background
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Name/Gene ID: NRF1
Family: Transcription factor
Synonyms: NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL - Gene ID
- 4899
- NCBI Accession
- NP_005002
- UniProt
- Q16656
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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