Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

NRF1 antibody (AA 360-409)

NRF1 Reactivity: Human, Rat, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio), Dog, Monkey, Chicken, Pig, Xenopus laevis WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN462197
  • Target See all NRF1 Antibodies
    NRF1 (Nuclear Respiratory Factor 1 (NRF1))
    Binding Specificity
    • 15
    • 9
    • 8
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 360-409
    Reactivity
    • 71
    • 43
    • 35
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio), Dog, Monkey, Chicken, Pig, Xenopus laevis
    Host
    • 68
    • 5
    Rabbit
    Clonality
    • 60
    • 13
    Polyclonal
    Conjugate
    • 36
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NRF1 antibody is un-conjugated
    Application
    • 60
    • 29
    • 14
    • 14
    • 12
    • 11
    • 6
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human NRF1
    No Cross-Reactivity
    Mouse (Murine)
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product NRF1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    NRF1 (Nuclear Respiratory Factor 1 (NRF1))
    Alternative Name
    NRF1 / NRF-1 (NRF1 Products)
    Synonyms
    ALPHA-PAL antibody, C87038 antibody, D6Ertd415e antibody, SC:BZ3C13.1 antibody, SC:bZ3C13.2 antibody, ube2h antibody, Nfe2l1_retired antibody, IBR/F antibody, NRF1 antibody, alpha-pal antibody, nrf-1 antibody, LOC100220087 antibody, nuclear respiratory factor 1 antibody, nuclear respiratory factor 1 S homeolog antibody, NRF1 antibody, Nrf1 antibody, nrf1 antibody, nrf1.S antibody
    Background
    Name/Gene ID: NRF1
    Family: Transcription factor

    Synonyms: NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL
    Gene ID
    4899
    NCBI Accession
    NP_005002
    UniProt
    Q16656
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
You are here:
Support