NRF1 antibody (AA 360-409)

Catalog No. ABIN462197

Product Details anti-NRF1 Antibody

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Binding Specificity: AA 360-409

Reactivity: Human, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Dog, Monkey, Chicken, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NRF1

No Cross-Reactivity: Mouse (Murine)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa360-409 of human NRF1 (Q16656, NP_005002). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1 / NRF-1 (NRF1 Products)

Synonyms: ALPHA-PAL antibody, C87038 antibody, D6Ertd415e antibody, SC:BZ3C13.1 antibody, SC:bZ3C13.2 antibody, ube2h antibody, Nfe2l1_retired antibody, IBR/F antibody, NRF1 antibody, alpha-pal antibody, nrf-1 antibody, LOC100220087 antibody, nuclear respiratory factor 1 antibody, nuclear respiratory factor 1 S homeolog antibody, NRF1 antibody, Nrf1 antibody, nrf1 antibody, nrf1.S antibody

Background: Name/Gene ID: NRF1
Family: Transcription factor

Synonyms: NRF1, EWG, NRF-1, Nuclear respiratory factor 1, ALPHA-PAL

Gene ID: 4899

NCBI Accession: NP_005002

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha