ST18 antibody (AA 899-948)

Catalog No. ABIN6741555

Product Details anti-ST18 Antibody

Target: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Binding Specificity: AA 899-948

Reactivity: Human, Mouse, Rabbit, Pig, Cow, Guinea Pig, Horse, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ST18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ST18

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit, Horse, Pig (100%) Rat, Dog, Chicken (92%) Mouse, Bovine, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa899-948 of human ST18 (O60284, NP_055497). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Bat, Rabbit, Horse, Pig (100%), Galago, Rat, Dog, Turkey, Zebra finch, Chicken, Platypus (92%), Mouse, Bovine, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ST18

Target: ST18 (ZNF387) (Zinc Finger Protein 387 (ZNF387))

Alternative Name: ST18 (ZNF387 Products)

Synonyms: AV348974 antibody, Myt3 antibody, NZF-3 antibody, Nzf3 antibody, mKIAA0535 antibody, r-MyT3 antibody, ZC2HC10 antibody, ZNF387 antibody, suppression of tumorigenicity 18 antibody, ST18, C2H2C-type zinc finger antibody, St18 antibody, ST18 antibody

Background: Name/Gene ID: ST18

Synonyms: ST18, KIAA0535, Zinc finger protein 387, ZC2HC10, ZNF387

Gene ID: 9705

NCBI Accession: NP_055497

UniProt: O60284