PRDM5 antibody (AA 36-85)

Catalog No. ABIN6741548

Product Details anti-PRDM5 Antibody

Target: PRDM5 (PR Domain Containing 5 (PRDM5))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PRDM5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human PRDM5 (Q9NQX1, NP_061169). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Dog, Turkey, Zebra finch, Chicken (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PRDM5

Target: PRDM5 (PR Domain Containing 5 (PRDM5))

Alternative Name: PRDM5 (PRDM5 Products)

Synonyms: PRDM5 antibody, BCS2 antibody, PFM2 antibody, 4432417F03Rik antibody, 6530401I24Rik antibody, AI197291 antibody, E130112L17Rik antibody, PR/SET domain 5 antibody, PR domain containing 5 antibody, PRDM5 antibody, prdm5 antibody, Prdm5 antibody

Background: Name/Gene ID: PRDM5

Synonyms: PRDM5, BCS2, PFM2, PR domain containing 5, PR domain-containing protein 5

Gene ID: 11107

NCBI Accession: NP_061169

UniProt: Q9NQX1