GSC antibody (AA 35-84)

Catalog No. ABIN6741539

Product Details anti-GSC Antibody

Target: GSC (Goosecoid Homeobox (GSC))

Binding Specificity: AA 35-84

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GSC

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (92%) Mouse, Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human GSC (P56915, NP_776248). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Tamarin (100%), Galago, Panda, Dog (92%), Elephant (91%), Mouse, Rat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GSC

Target: GSC (Goosecoid Homeobox (GSC))

Alternative Name: GSC / Goosecoid (GSC Products)

Background: Name/Gene ID: GSC

Synonyms: GSC, Goosecoid homeobox, Homeobox protein goosecoid, Goosecoid

Gene ID: 145258

NCBI Accession: NP_776248

UniProt: P56915