GATA6 antibody (C-Term)

Catalog No. ABIN462170

Product Details anti-GATA6 Antibody

Target: GATA6 (GATA Binding Protein 6 (GATA6))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GATA6

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse (100%) Sheep, Rabbit, Pig (92%) Rat, Bovine (85%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human GATA6 (Q92908, NP_005248). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Bat, Horse (100%), Sheep, Rabbit, Pig (92%), Rat, Panda, Bovine (85%), Guinea pig (84%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IF

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GATA6

Target: GATA6 (GATA Binding Protein 6 (GATA6))

Alternative Name: GATA6 (GATA6 Products)

Synonyms: AA410133 antibody, GATA-6 antibody, XGATA-6 antibody, XGATA6 antibody, gata6a antibody, gata6b antibody, pnr antibody, tGata6 antibody, gata6 antibody, gata-6 antibody, GATA-5 antibody, cb603 antibody, GATA binding protein 6 antibody, GATA binding protein 6 S homeolog antibody, GATA binding protein 6 L homeolog antibody, GATA6 antibody, Gata6 antibody, gata6 antibody, gata6.S antibody, gata6.L antibody

Background: Name/Gene ID: GATA6

Synonyms: GATA6, GATA-binding factor 6, GATA-binding protein 6, GATA binding protein 6, Transcription factor GATA-6

Gene ID: 2627

NCBI Accession: NP_005248

UniProt: Q92908